Recitation slides:

Class_2_Recetation_Weber_2022.pdf

Skills:

• Benchling
• DNA Isolation
• Gel electrophoresis
• PCR
• Restriction digest
• Sanger sequencing

In this module, we are going to learn the basic SynBio toolkit - enzymatic reactions. These techniques are all useful for a wide variety of applications, experiments, devices, and ideas.

Determining your blood type!

Skills:

• Making agarose gels
• DNA purification (commercial kit vs DIY)
• PCR
• Sequence analysis
• Setting up an enzymatic reaction (restriction digest)
• Running and analyzing a gel

Required Background Reading: